I don't know if I understood correctly, but if you are asking me whether I have changed any settings on the GPU since I started using the S3Drive app, my answer is no. I am not an expert on Hackintosh either (it was assembled for me).
I installed the app a few days ago and it worked the first time I used it. Upon opening, it asked for the 'Access key', the 'Secret access key', etc., and I was able to access my bucket. I uploaded some files, then shut down the computer, and upon restarting, S3Drive no longer connected as I mentioned in my previous messages, even after trying various suggestions you provided.
Today I tried reopening S3Drive and it asked for login/sign up. I registered, tried to access my bucket on Cubbit again, and was able to access and upload a test file. I logged out and then logged back in and for now, it is working. I don't know why.
Maybe it needed my sign-up to work?
I will do some tests in the coming days and if there are any problems, I will write to you again. In the meantime, thank you for your kindness and help